Summary
Type: SPE
Application #: 303950
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning: 3 mL methanol, then 3 mL water and at last 3 mL 0.01 mol/L sodium acetate buffer (pH 6); (Do not let run the column dry.)
Sample aspiration: The prepared sample is passed through the column by vacuum.
Washing: 3 mL 0.01 mol/L sodium acetate buffer (pH 6), then 6 mL water
Drying: under vacuum (5 min)
Elution: 5 mL acetonitrile
Evaporation: nitrogen stream (40 °C)
Reconstitution: 1 mL of HPLC eluent A
Authors: Krivohlavek, A. et al.
Source: J. Sep. Sci. 2005, 28, 1434–1439